Morphological and anatomical studies on in vitro grapevine (Vitis vinifera L. cv. Nebbiolo) plants by Low Temperature Scanning Electron Microscopy
نویسنده
چکیده
Morphological, anatomical and physiological features of in vitro plantlets are affected by the environmental conditions experienced. The high water content inside in vitro-formed tissues makes difficult to maintain the original characteristics of samples from in vitro plantlets during the preparation procedures for morphological and anatomical studies. In this paper, micropropagated Vitis vinifera L. cv. Nebbiolo plantlets were cryofixed and observed in a frozen-hydrated state by Low Temperature Scanning Electron Microscopy. This system enables to analyse biological structures in their natural hydrated state. The morphological and anatomical data obtained with this method were presented and discussed.
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